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A simple and practical solvent system selection strategy for high-speed countercurrent chromatography based on the
Xiaohan Zhu1,2, Pengcheng Li1, Jintian Tang1
1Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Department of Engineering Physics, Tsinghua University, Beijing 100084, P. R. China. caixu1.2019@tsinghua.org.cn.
Analytical Methods : Advancing Methods and Applications
|November 16, 2022
Summary
This study validates a high-performance liquid chromatography (HPLC) polarity model for selecting high-speed countercurrent chromatography (HSCCC) solvent systems. The model efficiently predicts compound polarity, streamlining solvent selection for effective separation and isolation of natural products.
Area of Science:
- Chromatography
- Analytical Chemistry
- Natural Product Isolation
Background:
- Solvent system selection is critical for high-speed countercurrent chromatography (HSCCC) separation efficiency.
- Compound polarity is a key factor influencing both HPLC analysis and HSCCC separation.
- The accuracy of the HPLC polarity parameter model requires extensive validation due to potential influences from experimental conditions.
Purpose of the Study:
- To validate the applicability and accuracy of the HPLC polarity parameter model for predicting compound polarity.
- To assess the impact of varying HPLC experimental conditions (flow rate, column, methanol concentration) on polarity calculations.
- To demonstrate the utility of the validated model in selecting HSCCC solvent systems for natural product isolation.
Main Methods:
- Shake-flask experiments and HPLC analysis were performed on 14 selected compounds.
- Compound polarities were calculated using the HPLC polarity parameter model.
- HSCCC solvent systems were selected based on the model's predictions for mangosteen peel and Hypericum sampsonii Hance extracts.
Main Results:
- HPLC analysis indicated negligible effects of flow rate and column type on calculated compound polarities.
- Methanol concentration in HPLC experiments showed a trend of variation in calculated polarities.
- Despite minor polarity differences between methods, partition coefficients (K) remained suitable for HSCCC (0.5 < K < 2.0).
- The model successfully guided the selection of HSCCC solvent systems, enabling the isolation of mangostin and quercetin.
Conclusions:
- The HPLC polarity parameter model is a fast, efficient, and accurate method for HSCCC solvent system selection.
- The model significantly improves efficiency and reduces experimental time for HSCCC method development.
- Predicted compound polarities are sufficient for meeting HSCCC separation requirements, requiring minimal HPLC analyses.
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