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Related Concept Videos

Proteomics01:33

Proteomics

7.7K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.7K

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Updated: Aug 21, 2025

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome
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Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome

Published on: March 7, 2014

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Overview and considerations in bottom-up proteomics.

Rachel M Miller1, Lloyd M Smith1

  • 1Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA. smith@chem.wisc.edu.

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|November 16, 2022
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Summary
This summary is machine-generated.

Mass spectrometry-based proteomics is vital for understanding cellular biology and disease. This review highlights advancements in bottom-up proteomics, including protein inference and post-translational modification discovery, for a more comprehensive proteome view.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Bioanalytical Chemistry

Background:

  • Proteins are fundamental to cellular functions in health and disease.
  • Understanding the proteome is key for cellular biology and clinical insights.
  • Mass spectrometry-based proteomics is the primary technique for proteome analysis.

Purpose of the Study:

  • To provide an overview of mass spectrometry-based proteomics.
  • To highlight emerging areas in bottom-up proteomics.
  • To advance comprehensive proteome analysis.

Main Methods:

  • Review of mass spectrometry-based proteomics.
  • Focus on bottom-up proteomics approaches.
  • Discussion of protein inference, alternative proteases, sample-specific databases, and PTM discovery.

Main Results:

  • Proteomics is a growing field with ongoing advancements.
  • Key developing areas in bottom-up proteomics are identified.
  • New methods enhance the ability to identify and quantify proteins.

Conclusions:

  • Continued advancements in proteomics are necessary for a complete proteome understanding.
  • Emerging techniques promise deeper insights into cellular processes and disease.
  • This review underscores the potential of novel bottom-up proteomics strategies.