Related Experiment Video
Updated: Aug 21, 2025
07:13
Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation
Published on: January 7, 2019
8.1K
Comparison mitochondrial DNA DAMPs changes in platelet prepared by apheresis and PRP
Azita Chegini1, Leila Vakili1, Shahram Samiee1
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
Summary
Mitochondrial DNA (DAMPs) levels change during platelet storage. Storage in solutions like SDP increases DAMPs, while PRP decreases them, impacting transfusion safety.
Area of Science:
- Transfusion Medicine
- Immunology
- Molecular Biology
Background:
- Mitochondrial DNA (DAMPs) are implicated in immune modulation.
- DAMPs may contribute to adverse transfusion reactions.
Purpose of the Study:
- To investigate the changes in mitochondrial DNA (DAMPs) during platelet storage.
- To compare DAMPs levels in platelet-rich plasma (PRP) and solution-dependent processing (SDP) products.
Main Methods:
- Cross-sectional study of 22 PRP and 14 SDP samples.
- Quantitative real-time PCR used to assess mtDNA DAMPs on storage days 1, 3, and 5.
- Statistical analysis using REST 2009 software and multiple t tests.
Main Results:
- SDP showed increased mtDNA DAMPs fold change over storage (1.3x days 1-3, 1.5x days 3-5, 2.1x days 1-5).
- PRP showed decreased mtDNA DAMPs fold change over storage (0.8x days 1-3, 0.6x days 3-5, 0.49x days 1-5).
Conclusions:
- Platelet preparation and processing methods significantly influence mtDNA DAMPs levels.
- Storage conditions impact the release and stability of mtDNA DAMPs in platelet products.
