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Protocol to evaluate hyperlipidemia in zebrafish larvae.

Keerthana Balamurugan1, Raghavender Medishetti1, Pallavi Rao2

  • 1Center for Innovation in Molecular and Pharmaceutical Sciences, Dr. Reddy's Institute of Life Sciences, Hyderabad, Telangana, India; Doctoral Student Registered with Manipal Academy of Higher Education, Manipal, Karnataka, India.

STAR Protocols
|November 17, 2022
PubMed
Summary

This study presents a zebrafish model for studying lipid accumulation and hyperlipidemia, offering an in vivo system for atherosclerosis research. Protocols cover lipid staining, assays, RNA isolation, and ImageJ analysis for comprehensive evaluation.

Keywords:
MetabolismModel organisms

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Area of Science:

  • Cardiovascular Research
  • Zebrafish Models
  • Lipid Metabolism

Background:

  • Hyperlipidemia is a major risk factor for atherosclerosis.
  • Developing reliable in vivo models is crucial for studying lipid disorders.
  • Zebrafish larvae offer a rapid and accessible model system.

Purpose of the Study:

  • To establish a zebrafish larval model for studying lipid accumulation.
  • To provide detailed protocols for creating and analyzing this model.
  • To facilitate research into hyperlipidemic conditions like atherosclerosis.

Main Methods:

  • Generation of a zebrafish larval model exhibiting lipid accumulation.
  • Staining techniques for visualizing lipids.
  • Lipid estimation assays and RNA isolation.
  • ImageJ analysis for larval dimensions and hyperlipidemia context.

Main Results:

  • Successful generation of a zebrafish larval model for lipid accumulation studies.
  • Detailed methodologies for lipid quantification and molecular analysis.
  • Demonstration of ImageJ's utility in assessing model parameters.

Conclusions:

  • The developed zebrafish model serves as a valuable in vivo system for hyperlipidemia research.
  • The provided protocols enable researchers to study atherosclerosis and related conditions.
  • Context-specific interpretation of results is advised for accurate application.