Related Experiment Video
Updated: Aug 21, 2025

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
Published on: October 30, 2017
An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal
Harshini Surendran1, Lalitha Soundararajan1, Vijay Bhaskar Reddy K1
1Eyestem Research, Centre for Cellular and Molecular Platforms (C-CAMP), Bengaluru, Karnataka 560065, India.
Abstract:
We present an optimized protocol for guided differentiation of retinal pigment epithelium (RPE) cells from human-induced pluripotent stem cells (iPSC). De novo-generated RPE cells are mature, polarized, and mimic the cellular and molecular profile of primary RPE; they are also suitable for in vivo cell transplantation studies. The protocol includes an enrichment step, making it useful for large-scale GMP manufacturing. RPE cells produced following this protocol are appropriate for cell replacement therapy for macular degeneration and disease modeling. For complete details on the use and execution of this protocol, please refer to Surendran et al. (2021).

