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TRPV3 inhibits colorectal cancer cell proliferation and migration by regulating the MAPK signaling pathway

Wei Yu1, Jieping Huang2, Hui Yu1

  • 1Department of Clinical Pharmacy, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, China.

Journal of Gastrointestinal Oncology
|November 17, 2022
PubMed
Abstract

Insights

Low expression of transient receptor potential vanilloid 3 (TRPV3) in colorectal cancer (CRC) is linked to poor prognosis. TRPV3 inhibits cancer cell proliferation and migration by suppressing the MAPK signaling pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Transient receptor potential vanilloid 3 (TRPV3) is a potential target in cancer therapy.
  • Colorectal cancer (CRC) is a significant global health concern with a need for improved prognostic markers.
  • Understanding the role of TRPV3 in CRC progression is crucial for developing novel treatment strategies.

Purpose of the Study:

  • To investigate the inhibitory effect of TRPV3 on colorectal cancer (CRC) cell proliferation and migration.
  • To explore the underlying molecular mechanisms, including signaling pathways involved.
  • To evaluate the prognostic value of TRPV3 expression in CRC patients.

Main Methods:

  • Analysis of a large microarray dataset (GSE39582) and validation using 100 CRC tissue samples.
  • In vitro functional assays: Cell Counting Kit-8 (CCK-8) for proliferation and Transwell assay for migration.
  • Gene Set Enrichment Analysis (GSEA) to identify regulated pathways, including the mitogen-activated protein kinase (MAPK) pathway.
  • In vivo experiments to confirm the role of TRPV3 and MAPK signaling.

Main Results:

  • Low TRPV3 expression is significantly associated with poor prognosis in CRC patients, validated at both gene and protein levels.
  • TRPV3 knockdown enhanced CRC cell proliferation and migration, while TRPV3 overexpression inhibited these processes.
  • TRPV3 silencing promoted CRC cell proliferation and migration by activating the MAPK signaling pathway.

Conclusions:

  • Low TRPV3 expression indicates a poor prognosis in colorectal cancer patients.
  • TRPV3 acts as an inhibitor of CRC cell proliferation and migration.
  • The mechanism involves the suppression of the MAPK signaling pathway by TRPV3.
Keywords:
Colorectal cancer (CRC)cell proliferation and migrationmitogen-activated protein kinase signaling pathway (MAPK signaling pathway)transient receptor potential vanilloid 3 (TRPV3)

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