Optimized gamma radiation produces physiological and morphological changes that improve seed yield in wheat

To study the effect of gamma radiation on various morphological and agronomic characters of bread wheat (Triticum aestivum L.), seeds were subjected to different gamma radiation doses; and selected M₅ and M₆ generation lines were evaluated. The optimum doses to induce desirable changes in bread wheat were 100-200 Gy. Seed loss decreased while grain yield, yield components, fertile florets number, biological yield, plant height, harvest index and flag leaf area increased in all mutant lines. Shear strength increased in many lines. Selected mutant lines also showed reduced seed shattering that can greatly reduce seed loss at harvest. Some new phenotypic characters such as the appearance of bristles on the glume, important for drought tolerance, two spikelets at each rachis and more fertile florets at each spikelet. These can greatly increase yield, as seen in some mutant lines. Also, some physiological characteristics including photosynthetic rate, stomatal conductance, water use efficiency, and chlorophyll content improved in some mutant lines. About 95.8% of the total variation in grain yield was explained by three selected variables: flag leaf area, number of seeds per spike, and spike number per plant. Grain yield increased more than 45% in some mutant lines the highest ever reported using this approach to the genetic improvement of wheat. Wheat grain yield has increased 2.2 times in the last 50 years, which indicates that if mutagens are optimally used and the selection is carefully performed as described herein, it is possible to improve important economic traits, in a much shorter time.

The online version contains supplementary material available at 10.1007/s12298-022-01225-0.