Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Mutational Landscape of Acute Myeloid Leukemia and Its Impact.

International journal of molecular sciences·2026

Same author

Cytokine inducible SH2-containing protein: a versatile negative regulator of cytokine receptor signaling.

Frontiers in immunology·2026

Same author

The Interleukin-7 Receptor Signaling Pathway and Its Perturbation in Immunodeficiency, Autoimmune Disease and Lymphoid Malignancy.

Biomolecules·2026

Same author

Colony-Stimulating Factor 3 Receptor Mutations and Variants in Hematological Malignancies.

Cancers·2025

Same author

Cytokine Receptor-like Factor 3 (CRLF3) and Its Emerging Roles in Neurobiology, Hematopoiesis and Related Human Diseases.

International journal of molecular sciences·2025

Same author

Socs3a is Dispensable for Zebrafish Hematopoiesis and is Required for Neuromast Formation.

Frontiers in bioscience (Landmark edition)·2025

Search research articles

Related Experiment Video

Updated: Aug 21, 2025

Isolating Malignant and Non-Malignant B Cells from lck:eGFP Zebrafish

Isolating Malignant and Non-Malignant B Cells from lck:eGFP Zebrafish

Published on: February 22, 2019

B cell lymphoma 6A regulates immune development and function in zebrafish.

Farooq L J Almohaisen^1,2, Somayyeh Heidary¹, Mohamed L Sobah¹

¹School of Medicine, Deakin University, Geelong, VIC, Australia.

Frontiers in Cellular and Infection Microbiology

|November 17, 2022

Summary

Zebrafish BCL6A (bcl6aa) is crucial for immune cell development and macrophage function. Loss of bcl6aa impairs macrophage mobility and bacterial infection containment, highlighting conserved roles in immunity.

Keywords:

BCL6A immunity lymphocyte macrophage zebrafish

More Related Videos

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

Published on: October 8, 2018

Author Spotlight: Identification and Isolation of Quiescent Leukemia Stem Cells from Zebrafish T-ALL

Author Spotlight: Identification and Isolation of Quiescent Leukemia Stem Cells from Zebrafish T-ALL

Published on: July 19, 2024

Related Experiment Videos

Last Updated: Aug 21, 2025

Isolating Malignant and Non-Malignant B Cells from lck:eGFP Zebrafish

Isolating Malignant and Non-Malignant B Cells from lck:eGFP Zebrafish

Published on: February 22, 2019

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

Published on: October 8, 2018

Author Spotlight: Identification and Isolation of Quiescent Leukemia Stem Cells from Zebrafish T-ALL

Author Spotlight: Identification and Isolation of Quiescent Leukemia Stem Cells from Zebrafish T-ALL

Published on: July 19, 2024

Area of Science:

Immunology
Developmental Biology
Genetics

Background:

BCL6A is a transcriptional repressor vital for lymphocyte development and survival.
BCL6A is highly expressed in non-Hodgkin's lymphomas.
Its role in non-hematopoietic cells, like macrophages, requires further study.

Purpose of the Study:

Identify and characterize zebrafish BCL6A (bcl6aa).
Investigate the role of bcl6aa in immune cell development and function, focusing on early macrophages.

Main Methods:

Bioinformatics analysis to identify zebrafish BCL6A homologues.
Zebrafish embryogenesis expression analysis.
Generation and analysis of bcl6aa homozygous and heterozygous mutants.
Assessment of immune cell populations, macrophage mobility, and bacterial infection response.

Main Results:

Identified zebrafish bcl6aa and bcl6ab homologues; human BCL6A and zebrafish Bcl6aa proteins show high domain conservation.
bcl6aa expression observed in early myeloid development sites and later in brain, eye, and thymus.
bcl6aa mutants exhibit decreased lymphoid and macrophage cells, with impaired macrophage mobility and bacterial containment in homozygous mutants.
Neutrophil numbers remained unaffected.

Conclusions:

Zebrafish bcl6aa is conserved with human BCL6A, particularly in key protein domains.
bcl6aa plays a significant role in zebrafish immune cell development, especially macrophages.
Loss of bcl6aa function negatively impacts macrophage-mediated immunity, suggesting conserved evolutionary roles.