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A simple method for the preparation and storage of helper T cells
Journal of Immunological Methods
|January 1, 1978
Abstract:
Relatively purified populations of helper T cells free of active PFC precursors can be prepared from primed splenocytes by irradiation, hypotonic shock, and filtration over glass wool. These purified populations can be stored and helper activity recovered undiminished after up to 6 months of cryopreservation.