Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple visual surface immunology test.

I Giaever

    Journal of Immunological Methods
    |January 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    A novel immunology test detects proteins at 1 microgram/ml. This method uses antigen-antibody reactions and plastic particles to create visible spots for easy detection.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Global, synchronous oscillations in cytosolic calcium and adherence in bradykinin-stimulated Madin-Darby canine kidney cells.

    Acta physiologica Scandinavica·2004
    Same author

    Correlated motion and oscillation of neighboring cells in vitro.

    Cell communication & adhesion·2002
    Same author

    Electrical impedance of cultured endothelium under fluid flow.

    Annals of biomedical engineering·2001
    Same author

    Electric cell-substrate impedance sensing (ECIS) as a noninvasive means to monitor the kinetics of cell spreading to artificial surfaces.

    Experimental cell research·2000
    Same author

    Histamine alters endothelial barrier function at cell-cell and cell-matrix sites.

    American journal of physiology. Lung cellular and molecular physiology·2000
    Same author

    Cell-substrate contact: another factor may influence transepithelial electrical resistance of cell layers cultured on permeable filters.

    Experimental cell research·1999
    Same journal

    Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters.

    Journal of immunological methods·2026
    Same journal

    Isosulfan blue sentinel lymph node biopsy enables reliable lymph node harvest and multicolor flow cytometry in mice.

    Journal of immunological methods·2026
    Same journal

    Type-specific antibody detection of herpes simplex virus types 1&2 (HSV-1&2) in fingerstick blood at point-of-care sites by a rapid and sensitive lateral flow immunochromatographic assay.

    Journal of immunological methods·2026
    Same journal

    Development of chimeric DGP-IgG antibodies as quality control for celiac disease diagnosis.

    Journal of immunological methods·2026
    Same journal

    Stepwise single-cell-resolved deep immunophenotyping pipeline to characterise immune heterogeneity and functionality in health and disease.

    Journal of immunological methods·2026
    Same journal

    Performance evaluation of Meso Scale Discovery (MSD) quantitative serological assays for detection of binding (IgG, IgA, IgM) and ACE2 inhibitory antibody levels for SARS-CoV-2.

    Journal of immunological methods·2026
    See all related articles

    Area of Science:

    • Immunology
    • Biochemistry
    • Analytical Chemistry

    Background:

    • Accurate protein detection is crucial for diagnostics.
    • Existing immunological assays can be complex and require specialized equipment.

    Purpose of the Study:

    • To develop a simple, sensitive, and general immunology test for protein detection.
    • To establish a method capable of detecting proteins in the low microgram/ml range.

    Main Methods:

    • Adsorbing antigen onto a glass surface.
    • Incubating with specific antibody solution.
    • Applying plastic particles that bind to protein layers.
    • Breaking antigen-antibody bonds with acid to reveal a visible spot.

    Main Results:

    Related Experiment Videos

    • The test successfully detects proteins at concentrations as low as 1 microgram/ml.
    • The method produces easily visible spots indicating the presence of the target protein.
    • The procedure is straightforward, involving adsorption, incubation, particle application, and acid treatment.

    Conclusions:

    • A new, sensitive general immunology test for protein detection has been developed.
    • The described method offers a simple and visible approach for immunological assays.
    • This technique has potential applications in various diagnostic and research settings.