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Updated: Aug 20, 2025

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches
Published on: October 8, 2021
High content design of riboswitch biosensors: All-around rational module-by-module design
Yifan Wu1, Longjiao Zhu1, Shuting Li1
1Food Laboratory of Zhongyuan, Key Laboratory of Precision Nutrition and Food Quality, Beijing Laboratory for Food Quality and Safety, Department of Nutrition and Health, China Agricultural University, Beijing, 100191, China.
Abstract:
Riboswitches are ideal, diverse regulatory elements with simple mechanisms. Therefore, they are widely employed in synthetic biology to construct highly efficient biosensors for biosensing and bioimaging. This review presents a rational riboswitch biosensor design that combines computational and hybrid methods. Four main steps are discussed, including the rational design of riboswitch screening libraries, rational riboswitch screening, rational tailoring, and the rational selection of reporting elements. Furthermore, the application of riboswitch biosensors for molecular detection and biological imaging is introduced. Finally, the future development and construction of ideal riboswitch biosensors are discussed, while assessing their application for point-of-care testing, health monitoring, and gene therapy. This review is expected to provide a comprehensive overview of the rational construction of riboswitch biosensors.
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