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Development of a PCR assay for the detection of human herpes virus type 7
S S Mardanly1, S G Mardanly1, A A Kazakov2
1CJSC «Ekolab».
Klinicheskaia Laboratornaia Diagnostika
|November 18, 2022
Abstract:
A PCR assay has been developed to identify the DNA of the human herpes virus type 7. The search and selection of conserved regions was carried out by comparing the whole genome nucleotide sequences of HHV-7. A fragment duplicated in the HHV-7 genomes was chosen as a target for amplification. The performance of the assay was tested on a synthetic matrix and clinical samples. The developed assay has high sensitivity and specificity and showed good efficiency in detecting HHV-7 DNA in clinical samples.
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