Bioinformatic analysis and clinical diagnostic value of hsa_circ_0004099 in acute ischemic stroke

Area of Science:

• Biochemistry
• Genetics
• Neuroscience

Background:

• Acute ischemic stroke (AIS) is a leading cause of death and disability worldwide.
• Identifying reliable biomarkers for AIS diagnosis and prognosis is crucial for effective treatment.
• Circular RNAs (circRNAs) are emerging as important regulators in various diseases, including neurological disorders.

Purpose of the Study:

• To investigate the expression and diagnostic value of hsa_circ_0004099 in patients with AIS.
• To explore the correlation between hsa_circ_0004099 levels and clinical parameters of AIS.
• To analyze the potential role of hsa_circ_0004099 in AIS pathogenesis using bioinformatics.

Main Methods:

• Case-controlled study involving 40 AIS patients and 40 healthy controls.
• Differential expression analysis using GEO2R and validation by RT-PCR.
• Correlation analysis with NIHSS scores and infarct time.
• Receiver operating characteristic (ROC) curve analysis for diagnostic accuracy.
• Bioinformatic analyses including KEGG and protein-protein interaction (PPI) network construction.

Main Results:

• hsa_circ_0004099 expression was significantly altered in AIS patients.
• NIHSS scores and infarct time showed negative correlations with hsa_circ_0004099 levels (r = -0.7053 and r = -0.5130, respectively).
• hsa_circ_0004099 demonstrated high diagnostic accuracy for AIS (AUC = 0.923).
• Bioinformatic analysis identified enrichment in cancer pathways and key proteins involved in AIS pathogenesis.

Conclusions:

• hsa_circ_0004099 serves as a potential clinical diagnostic marker for AIS.
• Its expression levels correlate with disease severity and infarct duration.
• hsa_circ_0004099 may play a role in AIS pathogenesis, offering a novel molecular target for therapeutic intervention.