Related Experiment Video
Updated: Aug 20, 2025

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
Published on: November 30, 2021
Genomic ancestry, diet and microbiomes of Upper Palaeolithic hunter-gatherers from San Teodoro cave
Gabriele Scorrano1,2, Sofie Holtsmark Nielsen3, Domenico Lo Vetro4,5
1Section for Evolutionary Genomics, Globe Institute, University of Copenhagen, Copenhagen, Denmark. g.scorrano@sund.ku.dk.
Abstract:
Recent improvements in the analysis of ancient biomolecules from human remains and associated dental calculus have provided new insights into the prehistoric diet and genetic diversity of our species. Here we present a multi-omics study, integrating metagenomic and proteomic analyses of dental calculus, and human ancient DNA analysis of the petrous bones of two post-Last Glacial Maximum (LGM) individuals from San Teodoro cave (Italy), to reconstruct their lifestyle and the post-LGM resettlement of Europe. Our analyses show genetic homogeneity in Sicily during the Palaeolithic, representing a hitherto unknown Italian genetic lineage within the previously identified Villabruna cluster. We argue that this lineage took refuge in Italy during the LGM, followed by a subsequent spread to central-western Europe. Analysis of dental calculus showed a diet rich in animal proteins which is also reflected on the oral microbiome composition. Our results demonstrate the power of this approach in the study of prehistoric humans and will enable future research to reach a more holistic understanding of the population dynamics and ecology.
Related Concept Videos
What is Evolutionary History?
The Fossil Record
Diversity of Archaea III
Diversity of Archaea IV
Diversity of Archaea II
Diversity of Archaea I

