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Design and characterization of a high-resolution multiple-SNP capture array by target sequencing for sheep
Yingwei Guo1, Fengting Bai1, Jintao Wang1
1Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Journal of Animal Science
|November 19, 2022
Summary
Researchers developed a low-cost ovine genotyping array using liquid chip technology. This array identified significant genetic loci for sheep hair type and teat number, advancing molecular breeding and genomic studies.
Area of Science:
- Animal Genomics
- Molecular Breeding
- Genotyping Technologies
Background:
- Efficient molecular breeding relies on cost-effective genotyping arrays.
- Developing high-resolution genotyping tools is crucial for livestock improvement.
Purpose of the Study:
- To develop an ovine high-resolution multiple-single-nucleotide polymorphism (SNP) capture array using genotyping by target sequencing (GBTS) and liquid chip technology.
- To identify genetic loci associated with sheep hair type and teat number.
Main Methods:
- Designed a 40K SNP capture array targeting important genomic regions in sheep.
- Employed capture-in-solution (liquid chip) technology for genotyping.
- Conducted genome-wide association studies (GWAS) on 317 sheep using the developed array.
Main Results:
- Identified over 210K high-quality SNPs with a 99.7% capture ratio for target regions.
- Detected a significant association for hair type on chromosome 25, implicating the IRF2BP2 gene.
- Discovered a novel candidate region on chromosome 13 associated with sheep teat number.
Conclusions:
- The developed liquid chip array provides high accuracy and low cost for ovine genotyping.
- This technology can accelerate genomic selection and breeding programs in sheep.
- Identified key genes and regions for important economic traits like hair type and teat number.
