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Flame Photometry: Lab01:16

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In a flame photometer, when a solution like potassium chloride is aspirated into the flame, the solvent evaporates, leaving behind dehydrated salt. This salt dissociates into free gaseous atoms in their ground state. Some of these atoms absorb energy from the flame, leading to their excitation. The excited atoms return to the ground state, emitting photons at characteristic wavelengths. Because only electronic transitions are involved, the resulting emission lines are very narrow. The intensity...
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Methodology for Sputum Induction and Laboratory Processing
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Fully integrated colorimetric sensor based on transparency substrate for salbutamol determination.

Atchara Lomae1, Sudkate Chaiyo2, Orawon Chailapakul1

  • 1Electrochemistry and Optical Spectroscopy Center of Excellence, Department of Chemistry, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.

Methodsx
|November 21, 2022
PubMed
Summary

A new colorimetric method determines salbutamol (SAL) using a portable, low-cost transparency-based analytical device (TAD) and potassium permanganate. This method offers accurate and precise SAL detection with a low limit of detection.

Keywords:
Colorimetric detectionPotassium permanganateRedox reactionSalbutamolTransparency-based analytical device

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Area of Science:

  • Analytical Chemistry
  • Chemical Sensing
  • Materials Science

Background:

  • Salbutamol (SAL) is a widely used bronchodilator.
  • Accurate and accessible methods for SAL determination are crucial for quality control and therapeutic monitoring.
  • Existing analytical methods may require sophisticated equipment or complex procedures.

Purpose of the Study:

  • To develop a facile and portable colorimetric method for salbutamol determination.
  • To utilize a transparency-based analytical device (TAD) for visual and quantitative SAL analysis.
  • To employ potassium permanganate as a cost-effective colorimetric reagent.

Main Methods:

  • Fabrication of a transparency-based analytical device (TAD) using wax-printing on a polymer substrate.
  • Development of a colorimetric assay based on the redox reaction between salbutamol and potassium permanganate in an acidified medium.
  • Visual observation of color change (light pink to orange) and quantitative analysis using digital camera and ImageJ software.

Main Results:

  • The developed method demonstrated a linear response for salbutamol detection in the range of 0.5 to 40 mg·L⁻¹.
  • A low limit of detection (LOD) of 0.05 mg·L⁻¹ was achieved.
  • The transparency-based analytical device (TAD) provided accurate and precise results for salbutamol determination.

Conclusions:

  • The proposed transparency-based analytical device (TAD) offers a simple, low-cost, and portable platform for salbutamol detection.
  • The permanganate-based colorimetric method exhibits excellent performance characteristics, including good accuracy and high precision.
  • This method holds potential for rapid and on-site determination of salbutamol.