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Updated: Aug 20, 2025

3D Visualization of Retinal Vascular Pericytes in Mice by Immunostaining
Published on: November 1, 2024
Transforming Confocal Microscopy Imaging Data of Retinal Vasculature into 3D Animation
Emilyn Frohn1, Leah Lebowicz1, Rex Twedt1
1Biomedical Visualization Graduate Program, University of Illinois at Chicago, Chicago, Illinois.
Abstract:
This research study presents a methodology for creation of 3D models from confocal microscopy, specifically the retinal trilaminar capillary network, that can be used in 3D biomedical animation. Biomedical visualization professionals regularly use CT and MRI imaging data to create 3D models and other visuals. Adding confocal microscopy to this toolkit using ImageJ FIJI1 and Materialise Mimics® allows for a wider range of options to create data-driven 3D models of microscopic structures. Understanding the interactions of the neurovascular unit through visualization may be a key to advancing understanding of normal function of the retina and diabetic retinopathy pathology. This Vesalius Trust research poster was presented at the Association of Medical Illustrators' 2021 virtual annual meeting.
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