Nickel-Cobalt Oxide Nanoparticle-Induced Biohydrogen Production

The positive effects of metal oxide nanoparticles (NPs) on dark fermentation (DF) for biohydrogen synthesis have been increased, and the mechanism still needs to be further revealed. In this study, nickel-cobalt oxide (NiCo₂O₄) NPs were prepared to increase H₂ yield via DF. The highest (259.67 mL/g glucose) and the lowest (188.14 mL/g glucose) yields were achieved at 400 and 800 mg/L NiCo₂O₄ NPs added, respectively, with their corresponding 33.97% increase and 2.93% decrease compared with the control yield (193.82 mL/g glucose). Meanwhile, the microbial community further confirmed that NiCo₂O₄ NPs increased the abundance of the dominant H₂-producing Clostridium sensu stricto 1 by 23.05%. The gene prediction also showed that NiCo₂O₄ NPs increased the abundance of genes encoding the rate-limiting enzyme pyruvate kinase in glycolysis, thus increasing the substrate conversion. Moreover, the gene abundance of key enzymes directly related to H₂ evolution was also increased at different levels.