Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Model for DNA packaging into bacteriophage T4 heads.

L W Black, D J Silverman

    Journal of Virology
    |November 1, 1978
    PubMed
    Summary

    Bacteriophage T4 DNA packaging involves linear, unidirectional entry into the head, with the first DNA end attaching to the capsid. Proteins p20 and p17 enzymatically pump and twist the DNA into the head.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Selective activation of signal transducer and activator of transcription (STAT) proteins STAT1 and STAT3 in human endothelial cells infected with Rickettsia rickettsii.

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2009
    Same author

    Rickettsia rickettsii infection of human pulmonary microvascular endothelial cells: modulation of cyclooxygenase-2 expression.

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2009
    Same author

    Quantitative analyses of variations in the injury of endothelial cells elicited by 11 isolates of Rickettsia rickettsii.

    Clinical and diagnostic laboratory immunology·2001
    Same author

    Green fluorescent protein as a probe of rotational mobility within bacteriophage T4.

    Journal of virological methods·2000
    Same author

    Interaction of rickettsiae with eukaryotic cells. Adhesion, entry, intracellular growth, and host cell responses.

    Sub-cellular biochemistry·2000
    Same author

    Post-transcriptional regulation of endothelial cell plasminogen activator inhibitor-1 expression during R. rickettsii infection.

    Microbial pathogenesis·2000

    Area of Science:

    • Molecular Biology
    • Virology
    • Genetics

    Background:

    • Bacteriophage T4 is a model organism for studying DNA packaging.
    • Understanding DNA packaging is crucial for viral replication and gene therapy vectors.

    Purpose of the Study:

    • To elucidate the in vivo mechanism of DNA packaging into bacteriophage T4 heads.
    • To investigate the structure and orientation of DNA within the phage head.

    Main Methods:

    • Glucosylation of hydroxymethylcytosine residues in a conditionally glucose-deficient host.
    • Temperature shift experiments with temperature-sensitive mutant ts49.
    • Restriction enzyme digestion and autoradiography of mature phage DNA.

    Main Results:

    • Cytoplasmic DNA is glucosylated, but DNA within mature phage heads is resistant.
    • Mature T4 phage DNA is partially glucosylated, suggesting linear, unidirectional packaging.
    • The first DNA end enters the capsid without sequence specificity and is deposited centrally.

    Conclusions:

    • DNA is packaged linearly and unidirectionally into the bacteriophage T4 head.
    • Proteins p20 and p17 likely mediate DNA packaging through enzymatic action and torsion induction.
    • The packaging mechanism provides insights into DNA organization within viral capsids.

    Related Experiment Videos