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Mycobacteriophage Tarkin: a Cluster E Phage.

Katherine E Cleary¹, Anna M Fakhri¹, Ethan N Dionne¹

¹Department of Chemistry and Biochemistry, Providence College, Providence, Rhode Island, USA.

Microbiology Resource Announcements

|November 21, 2022

Summary

Mycobacteriophage Tarkin, a novel phage infecting Mycobacterium smegmatis, has a 75,998-bp genome. This phage is classified into cluster E based on its gene content similarity to other mycobacteriophages.

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Area of Science:

Microbiology
Virology
Genomics

Background:

Mycobacteriophages are viruses that infect mycobacteria.
Mycobacterium smegmatis is a commonly studied non-pathogenic species used in phage research.

Purpose of the Study:

To characterize a newly isolated mycobacteriophage, named Tarkin.
To determine the genomic features and phylogenetic placement of Mycobacteriophage Tarkin.

Main Methods:

Isolation and characterization of a novel mycobacteriophage.
Whole-genome sequencing of Mycobacteriophage Tarkin.
Bioinformatic analysis of the genome for gene content and phylogenetic grouping.

Main Results:

Mycobacteriophage Tarkin was isolated and infects Mycobacterium smegmatis mc2155.
The genome sequence of Tarkin is 75,998 base pairs.
Tarkin is predicted to contain 142 protein-coding genes and 2 tRNA genes.
Phylogenetic analysis based on gene content places Tarkin within cluster E of mycobacteriophages.

Conclusions:

Mycobacteriophage Tarkin represents a new addition to the known diversity of mycobacteriophages.
The genomic characteristics of Tarkin are consistent with its classification in cluster E.
Further studies on Tarkin can contribute to understanding mycobacteriophage biology and evolution.