Related Experiment Video
Updated: Aug 20, 2025
Use of Rabbit Eyes in Pharmacokinetic Studies of Intraocular Drugs
Published on: July 23, 2016
In-vivo inhibition of latanoprost induced iridal hyperpigmentation in rabbits- An investigational study
Muhammad Sadiq1, Waqar Ahmad2, Muhammad Bilal3
1Department of Pharmacy, University of Malakand, Chakdara, Pakistan.
Objectives:
To evaluate the inhibitory effects of different concentrations of α-methyl-DL-tyrosine on latanoprost-induced iridal hyperpigmentation in rabbits.
Methods:
We investigated 4 groups of rabbits. Both eyes of the pink, red, and blue groups were treated with latanoprost followed by 0.5%, 1%, and 2% α-methyl-DL-tyrosine (inhibitor) in the right eyes respectively and the green group received only inhibitor. We prospectively investigated the irides, estimated quantitatively total melanin contents, and studied any histopathological changes that occurred.
Results:
The observers favored hyperpigmentation in the left eyes while in the right eyes they noted a decrease in pigmentation as compared to the baseline. An increase in pigmentation was noted by 93.33% of observers in the left eye of the blue group. A significant difference in the mean melanin contents was noted in the blue group (Right eye = 09.560 μg/g (±0.750), Left eye = 3.730 μg/g (±1.062). There was no evidence of stromal malignant changes, Hemorrhage, mitosis, inflammation, and atypical melanocytes in all specimens. A moderate degree of pigmentation in the left eye of the red group was noted. Mild stromal-free melanin pigment was present in all samples of pink, red and blue groups.
Conclusions:
The α-methyl-DL-tyrosine significantly inhibited latanoprost-induced iridal pigmentation without causing any histopathological changes at a 2% dose.
