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Updated: Aug 20, 2025

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Recent Progress and Challenges on the Microfluidic Assay of Pathogenic Bacteria Using Biosensor Technology.

Farnaz Bahavarnia1,2, Mohammad Hasanzadeh2,3, Deniz Sadighbayan4

  • 1Jiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources and International Innovation Center for Forest Chemicals and Materials, Nanjing Forestry University, Nanjing 210037, China.

Biomimetics (Basel, Switzerland)
|November 22, 2022
PubMed
Summary

Microfluidic technology enables rapid and cost-effective detection of pathogenic bacteria using nanofluidic biosensing. This review highlights advanced nanomaterials and methods for sensitive pathogen recognition in biological samples.

Keywords:
advanced nanomaterialbiomedical analysisclinical infectionsmicrofluidicpathogen

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Microfluidics

Background:

  • Microfluidic technology leverages micro/nanoliter volumes for reduced test costs and duration.
  • It is widely applied in biological and medical research and diagnostics.
  • Miniaturized chips with microscale channels and chambers are central to this technology.

Purpose of the Study:

  • To review the significance and applications of nanofluidic biosensing for pathogenic bacteria detection.
  • To investigate microfluidic devices integrated with bioreceptors and nanomaterials.
  • To critically assess microfluidic methods for sensitive and selective pathogen recognition.

Main Methods:

  • Survey of microfluidic methods for pathogenic bacteria recognition.
  • Analysis of microfluidic devices incorporating bioreceptors and nanomaterials (e.g., nano-polymers, carbon nanomaterials, hydrogels, noble metals).
  • Evaluation of advantages and limitations of different recognition methods.

Main Results:

  • Microfluidic applications show significant potential for pathogenic bacteria detection.
  • Integration of advanced nanomaterials enhances biosensing capabilities.
  • Various microfluidic methods offer sensitive and selective pathogen recognition.

Conclusions:

  • Microfluidic-based nanofluidic biosensing is a promising approach for diagnosing pathogenic bacterial infections.
  • Future research opportunities lie in optimizing these systems for clinical applications.
  • Continued development can lead to improved disease diagnosis and management.