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Related Experiment Video

Updated: Aug 20, 2025

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

Published on: December 9, 2022

Decoding Single-cell Landscape and Intercellular Crosstalk in the Transplanted Liver.

Haitao Huang^1,2, Ruihan Chen^1,2, Yimou Lin^1,2

¹Department of Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Transplantation

|November 22, 2022

Summary

This study reveals the cellular dynamics of transplanted livers, showing immune cell replacement and the potential for recipient cells to become tissue-resident. This provides insights into graft recovery and crosstalk after liver transplantation (LT).

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Last Updated: Aug 20, 2025

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Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Published on: February 18, 2017

Area of Science:

Immunology
Transplantation Biology
Single-cell Genomics

Background:

Liver transplantation (LT) is a critical treatment for end-stage liver disease.
Understanding graft reconstruction and cellular crosstalk post-LT remains challenging due to biological complexity.

Purpose of the Study:

To investigate the physiological processes of graft recovery and intercellular crosstalk following LT.
To create a detailed cellular atlas of the transplanted liver.

Main Methods:

Establishment of an immune-tolerated orthotopic LT mouse model.
Application of single-cell RNA sequencing and CyTOF for comprehensive cellular analysis.

Main Results:

Identification of acute and stable phases in perioperative graft recovery.
Detailed annotation of the transplanted liver's cellular landscape and dynamic cell modifications.
Discovery that recipient immune cells largely replace graft cells, with notable exceptions of specific macrophage and natural killer cell populations exhibiting tissue-resident potential.

Conclusions:

Delineation of a comprehensive cell atlas (type, proportion, source, time) for the transplanted liver.
Elucidation of graft reconstruction mechanisms and graft-recipient cellular crosstalk.
Highlighting the potential for recipient-derived cells to differentiate into tissue-resident populations post-LT, influencing liver remodeling.