Expression, Purification, and Enzymatic Analysis of Plant SUMO Proteases
Prakash Kumar Bhagat1, Dipan Roy1, Ari Sadanandom2
1Department of BioSciences, University of Durham, Durham, UK.
Methods in Molecular Biology (Clifton, N.J.)
|November 22, 2022
Abstract:
The conjugation of SUMO can profoundly change the behavior of substrate proteins, impacting a wide variety of cellular responses. SUMO proteases are emerging as key regulators of plant adaptation to its environment because of their instrumental role in the SUMO deconjugation process. Here we describe how to express, purify, and determine SUMO deconjugation activity of a plant SUMO protease.
