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Conformationally Flexible Dimeric-Serotonin-Based Sensitive and Selective Electrochemical Biosensing Strategy for
Sachin Ganpat Chavan1, Ajay Kumar Yagati1, Aneesh Koyappayil1
1School of Integrative Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-Gu, Seoul06974, South Korea.
Analytical Chemistry
|November 22, 2022
Summary
A new electrochemical sensor detects serotonin using a novel enzyme-mediated reaction. This low-cost, highly sensitive method enables point-of-care testing for this key neurotransmitter.
Area of Science:
- Electrochemistry
- Biomedical Engineering
- Neuroscience
Background:
- Serotonin is a crucial neurotransmitter involved in various physiological processes.
- Accurate and sensitive detection of serotonin is vital for diagnosing neurological and psychiatric disorders.
- Existing detection methods often lack the sensitivity, specificity, or cost-effectiveness required for point-of-care applications.
Purpose of the Study:
- To develop a novel electrochemical sensor for sensitive and specific serotonin detection.
- To explore the potential of a multi-functional nano-platform for biomedical sensing applications.
- To demonstrate a cost-effective and straightforward analytical method for neurotransmitter quantification.
Main Methods:
- Construction of an electrochemical sensor utilizing an enzyme-mediated reaction of serotonin per-oxidation.
- Formation of a 4,4'-dimeric-serotonin self-assembled derivative on a gold surface electrode.
- Exploration of the bivalent interaction strategy of the dimer for enhanced recognition.
- Quantification of serotonin using its quenching effect on the gold surface electrode system.
Main Results:
- The developed sensor demonstrated high specificity and sensitivity for serotonin detection.
- A low limit of detection (LOD) of 0.9 nM in phosphate buffer and 1.4 nM in human serum was achieved.
- A linear detection range of 10 to 400 nM with a sensitivity of 2.0 × 10-2 nM was established.
- The sensor exhibited excellent stability and reproducibility, suitable for point-of-care testing.
Conclusions:
- The novel electrochemical sensor based on serotonin dimerization offers a robust, low-cost, and highly sensitive platform for serotonin biosensing.
- This approach provides a promising new analytical method for detecting low concentrations of serotonin, particularly for point-of-care applications.
- The self-assembling gold surface electrochemical system represents a significant advancement in neurotransmitter detection technology.
