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Updated: Aug 20, 2025
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Published on: January 9, 2019
General Characteristics and Promotion Properties of Circular PLOD2 in Patients with Glioma
Hui Wu1, Lilin Cheng2, Wenhua Sun2
1Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Jiangsu, China; Department of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Background:
Circular RNAs are closed endogenous RNAs that are involved in the progression of diverse tumors. Even with the most advanced combined treatments, patients with glioblastoma multiforme have a median survival time of <15 months. This study aimed to investigate the roles of circular PLOD2 (circPLOD2) in glioma tumorigenesis and tumor development and to clarify its tumor-promoting effects by bioinformatics analysis and molecular experiments.
Methods:
To determine the characteristics of circPLOD2 expression, quantitative real-time polymerase chain reaction was conducted. Stable knockdown of circPLOD2 was implemented for functional assays. Cell Counting Kit-8 and colony formation assays were used to measure cell proliferation. Transwell assays and tube formation assays were used to evaluate cell invasion and angiogenesis abilities, respectively. An intracranial xenograft model was established to determine the function of circPLOD2 in vivo. Further biochemical and Western blot analyses were conducted to evaluate proteins associated with circPLOD2.
Results:
circPLOD2 was upregulated in glioma tissues and cells. High expression of circPLOD2 was significantly associated with tumor size, World Health Organization grade, and molecular characteristics of glioma. circPLOD2 deregulation affected glioblastoma multiforme cell proliferation, invasion, and angiogenesis. Knockdown of circPLOD2 inhibited tumorigenesis in vivo. Further biochemical analysis showed that circPLOD2 was involved in oncogenic pathways and correlated with the expression of proteins related to proliferation, invasion, and angiogenesis.
Conclusions:
Our data indicate that circPLOD2 promotes glioma tumorigenesis and tumor development in vitro and in vivo and that suppressing circPLOD2 could be a novel therapeutic strategy for glioma.
Insights
Circular PLOD2 (circPLOD2) is upregulated in glioma, promoting tumor growth, invasion, and angiogenesis. Suppressing circPLOD2 offers a potential new therapeutic strategy for glioma patients.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Glioblastoma multiforme (GBM) has a poor prognosis despite advanced treatments.
- Circular RNAs (circRNAs) are implicated in various cancer progressions.
- The role of circPLOD2 in glioma tumorigenesis requires further investigation.
Purpose of the Study:
- To investigate the role of circular PLOD2 (circPLOD2) in glioma development.
- To elucidate the tumor-promoting effects of circPLOD2 using bioinformatics and molecular experiments.
Main Methods:
- Quantitative real-time polymerase chain reaction (qRT-PCR) for circPLOD2 expression analysis.
- Functional assays including cell proliferation, invasion, and angiogenesis assays.
- In vivo studies using an intracranial xenograft model and biochemical analyses.
Main Results:
- circPLOD2 was significantly upregulated in glioma tissues and cells.
- High circPLOD2 expression correlated with tumor size, WHO grade, and glioma molecular characteristics.
- circPLOD2 knockdown inhibited glioma cell proliferation, invasion, angiogenesis, and tumorigenesis in vivo.
Conclusions:
- circPLOD2 promotes glioma tumorigenesis and development both in vitro and in vivo.
- Targeting circPLOD2 represents a potential novel therapeutic strategy for glioma.
