Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Enzyme Kinetics01:19

Introduction to Enzyme Kinetics

20.3K
Enzyme kinetics studies the rates of biochemical reactions. Scientists monitor the reaction rates for a particular enzymatic reaction at various substrate concentrations. Additional trials with inhibitors or other molecules that affect the reaction rate may also be performed.
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
20.3K
Introduction to Mechanisms of Enzyme Catalysis01:13

Introduction to Mechanisms of Enzyme Catalysis

8.4K
For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes...
8.4K
Enzyme Kinetics01:19

Enzyme Kinetics

97.7K
Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
97.7K
Enzymes02:34

Enzymes

82.2K
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
82.2K
Induced-fit Model01:13

Induced-fit Model

81.4K
Most chemical reactions in cells require enzymes—biological catalysts that speed up the reaction without being consumed or permanently changed. They reduce the activation energy needed to convert the reactants into products. Enzymes are proteins, that usually work by binding to a substrate—a reactant molecule that they act upon.
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical...
81.4K
Introduction to Enzymes01:22

Introduction to Enzymes

19.0K
The use of enzymes by humans dates to 7000 BCE. Humans first used enzymes to ferment sugars and produce alcohol without knowing that this was an enzyme-catalyzed reaction. Wilhelm Kuhne coined the term 'enzyme' in 1877 from the Greek words ‘en’ meaning ‘in’ or ‘within’ and ‘zyme’ meaning ‘yeast.’
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that...
19.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Building an oral peptide drug.

Science (New York, N.Y.)·2026
Same author

Network-based allosteric analysis of galectin-7: Key residues dictate functional communication and stability.

Protein science : a publication of the Protein Society·2026
Same author

Indigo Formation as a Predictor of Non-Native Aromatic Hydroxylation in Cytochrome P450 BM3.

ACS catalysis·2026
Same author

Profile, Infection, and Vaccination Uptake: A Cohort of Canadian Retail Workers During the SARS-CoV-2 Pandemic.

Infectious disease reports·2025
Same author

From Binding to Catalysis: Emergence of a Rudimentary Enzyme Conferring Intrinsic Antibiotic Resistance.

Molecular biology and evolution·2025
Same author

Development and Use of a Galectin-1-Specific Nanobody for Tumor Imaging and Elucidating the Role of Galectin‑1 in Cancer.

ACS pharmacology & translational science·2025
Same journal

Combining bacterial display and protein language models to engineer a CD69-binding affibody for molecular imaging of immune activation.

Protein engineering, design & selection : PEDS·2026
Same journal

Examining selection dynamics and limitations in multi-round protein selection of high diversity libraries.

Protein engineering, design & selection : PEDS·2026
Same journal

A photo-enhanced oxidative coupling for site-specific protein Labeling via noncanonical amino acid incorporation.

Protein engineering, design & selection : PEDS·2026
Same journal

Engineering affibody domains as anti-idiotypic masks for nivolumab-based prodrugs.

Protein engineering, design & selection : PEDS·2026
Same journal

Integrating machine learning tools in protein design: a case of MHETase engineering for PET biodeconstruction.

Protein engineering, design & selection : PEDS·2026
Same journal

Computational redesign of a thermostable T7 RNA polymerase.

Protein engineering, design & selection : PEDS·2026
See all related articles

Related Experiment Video

Updated: Aug 20, 2025

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
09:42

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Published on: January 16, 2016

9.1K

Integrating dynamics into enzyme engineering.

Claudèle Lemay-St-Denis1,2,3, Nicolas Doucet1,4, Joelle N Pelletier1,2,3,5

  • 1PROTEO, The Québec Network for Research on Protein, Function, Engineering and Applications, Quebec, QC, Canada.

Protein Engineering, Design & Selection : PEDS
|November 23, 2022
PubMed
Summary
This summary is machine-generated.

Enzyme engineering is advancing, with new research linking protein dynamics to enzyme function. This opens doors for

Keywords:
conformational landscapeenzyme engineeringmolecular dynamicsprotein designprotein motions

More Related Videos

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis
08:49

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis

Published on: June 20, 2025

461
Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
10:28

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

9.0K

Related Experiment Videos

Last Updated: Aug 20, 2025

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
09:42

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Published on: January 16, 2016

9.1K
Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis
08:49

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis

Published on: June 20, 2025

461
Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
10:28

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

9.0K

Area of Science:

  • Biochemistry and Molecular Biology
  • Enzyme Engineering
  • Protein Dynamics

Background:

  • Enzyme engineering is a common practice in research and industry.
  • Significant progress has been made in characterizing protein dynamics using various methods.
  • Established links exist between protein dynamics and enzyme function.

Purpose of the Study:

  • To review the intersection of enzyme engineering and protein dynamics.
  • To identify challenges in the field.
  • To highlight pioneering research in dynamic engineering.

Main Methods:

  • Literature review of enzyme engineering studies.
  • Analysis of methodologies for protein dynamics characterization.
  • Examination of studies linking protein dynamics to enzyme function and engineering.

Main Results:

  • Characterizing enzyme dynamics before and after engineering informs potential applications.
  • The concept of 'dynamic engineering' is emerging, focusing on modifying protein dynamics.
  • Pioneering work demonstrates the feasibility of altering enzyme function through dynamic modifications.

Conclusions:

  • Understanding protein dynamics is crucial for advanced enzyme engineering.
  • Dynamic engineering offers a rational approach to alter enzyme function.
  • Further research is needed to overcome challenges and fully exploit dynamic engineering.