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Genome Sequence of a Lytic Staphylococcus aureus Bacteriophage Isolated from Breast Milk

Joshua J Iszatt1,2, Alexander N Larcombe1,2, Luke W Garratt1,2

  • 1Occupation, Environment and Safety, School of Population Health, Curtin University, Perth, Western Australia, Australia.

Microbiology Resource Announcements
|November 23, 2022
PubMed

Insights

Researchers discovered a novel double-stranded DNA bacteriophage, Biyabeda-mokiny 1, in breast milk. This finding expands our understanding of bacteriophages associated with Staphylococcus aureus infections.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Bacteriophages are viruses that infect bacteria and play crucial roles in microbial ecosystems.
  • The Herelleviridae family includes large, double-stranded DNA (dsDNA) bacteriophages.
  • Staphylococcus aureus is a common human pathogen.

Purpose of the Study:

  • To identify and characterize novel bacteriophages.
  • To investigate bacteriophages associated with Staphylococcus aureus.
  • To determine the genomic features of a newly isolated bacteriophage.

Main Methods:

  • Isolation of bacteriophage from breast milk using Staphylococcus aureus as a host.
  • Genomic DNA extraction and sequencing.
  • Bioinformatic analysis of the viral genome.

Main Results:

  • Identification of a dsDNA bacteriophage, named Biyabeda-mokiny 1.
  • Phylogenetic analysis suggests Biyabeda-mokiny 1 belongs to the Kayvirus genus within Herelleviridae.
  • The bacteriophage genome is 141,091 base pairs long and contains 230 putative coding sequences.

Conclusions:

  • Biyabeda-mokiny 1 represents a new member of the Kayvirus genus.
  • This bacteriophage was successfully isolated from human breast milk.
  • The genomic data provides insights into the diversity of Herelleviridae phages.

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