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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
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[Next-generation sequencing in ophthalmology].

Julian Wolf1,2,3, Clemens Lange1,2,4, Thomas Reinhard1,2

  • 1Klinik für Augenheilkunde, Universitätsklinikum Freiburg, Killianstr. 5, 79106, Freiburg, Deutschland.

Die Ophthalmologie
|November 23, 2022
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing (NGS) advances ophthalmology research by analyzing gene activity. RNA sequencing offers a comprehensive view of cellular gene expression, aiding in hypothesis generation and developing new diagnostics and treatments.

Keywords:
DNA sequencingGenomeMacular degenerationRNA sequencingTranscriptome analysis

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Area of Science:

  • Molecular Biology
  • Genomics
  • Ophthalmology

Context:

  • Emerging molecular biology techniques are increasingly integrated into ophthalmology.
  • Next-generation sequencing (NGS) offers powerful tools for basic research in eye diseases.

Purpose:

  • To introduce the principles of NGS and its application in ophthalmology.
  • To highlight the utility of transcriptome analysis via RNA sequencing in ocular research.

Summary:

  • This article explains NGS and focuses on RNA sequencing for transcriptome analysis in ophthalmology.
  • RNA sequencing provides a comprehensive, unbiased overview of gene activity in ocular cells and tissues.
  • This facilitates the generation of testable hypotheses and detailed characterization of pathological changes.

Impact:

  • RNA sequencing supports the development of novel diagnostic and therapeutic strategies for eye conditions.
  • It enhances the understanding of disease mechanisms at a molecular level.
  • This research method provides a foundation for future advancements in ophthalmic medicine.