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Updated: Aug 20, 2025

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
Bioinformatic Approaches for Describing the Oral Microbiota
Kristi Biswas1, Michael W Taylor2, David T J Broderick3,2
1Department of Surgery, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand. k.biswas@auckland.ac.nz.
Abstract:
Cultivation-independent (molecular) analysis of the oral microbiota can provide a comprehensive picture of microbial community composition, yet there is an at-times bewildering array of approaches that can be employed. This chapter introduces some of the key considerations when undertaking microbiota research and describes two alternative bioinformatic pipelines for conducting such studies. The descriptions are based on analysis of bacterial 16S ribosomal RNA gene sequences, but can be easily adapted for analysis of other microbial taxa such as fungi.
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