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Published on: February 2, 2024
Analysis of long non-coding RNA expression profile of bovine monocyte-macrophage infected by Mycobacterium avium
Yanhong Bao1, Shuiyin Wu1, Tianze Yang1
1College of Life Sciences, Jilin Agricultural University, Xincheng Street No.2888, Changchun, 130118, China.
Abstract:
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis. As a potential zoonotic pathogen, MAP also seriously threatens human health and social security. At present, long non-coding RNA (lncRNA) has attracted wide attention as an useful biomarker in various diseases. Therefore, our study analyzed the lncRNA expression profiles and lncRNA-mRNA regulatory network of MAP infected bovine monocytes-macrophages and uninfected bovine cells by high-throughput sequencing. A total of 4641 differentially expressed lncRNAs genes were identified, including 3111 up-regulated genes and 1530 down-regulated genes. In addition, lncRNA-mRNA interaction analysis was performed to predict the target genes of lncRNA. Among them, after MAP infection, 86 lncRNAs targeted to mRNA, of which only 6 genes were significantly different. The results of Gene Ontology (GO) enrichment analysis showed that the differentially expressed genes significantly enriched in functional groups were related to immune regulation. Multiple signal pathways including NF-κB, NOD-like receptor, Cytokine-cytokine receptor, Toll-like receptor signaling pathway, Chemokine signaling pathway, and other important biochemical, metabolic and signal transduction pathways were enriched in Kyoto Encyclopedia of Genes and Genomes (KEGG). In this study, analysis of macrophage transcriptomes in response to MAP infection is expected to provide key information to deeply understand role of the pathogen in initiating an inappropriate and persistent infection in susceptible hosts and molecular mechanisms that might underlie the early phases of paratuberculosis.
Insights
Mycobacterium avium subsp. paratuberculosis (MAP) infection alters long non-coding RNA (lncRNA) expression in bovine cells. This study reveals key lncRNA-mRNA networks involved in immune regulation during MAP infection.
Area of Science:
- Veterinary Immunology
- Molecular Biology
- Genomics
Background:
- Mycobacterium avium subsp. paratuberculosis (MAP) causes paratuberculosis, a disease impacting livestock and potentially humans.
- Long non-coding RNAs (lncRNAs) are emerging as critical biomarkers in various diseases.
- Understanding host-pathogen interactions at the molecular level is crucial for disease control.
Purpose of the Study:
- To investigate lncRNA expression profiles in bovine monocytes-macrophages following MAP infection.
- To construct and analyze the lncRNA-mRNA regulatory network in response to MAP.
- To identify molecular mechanisms underlying paratuberculosis pathogenesis.
Main Methods:
- High-throughput sequencing of lncRNA expression profiles.
- Bioinformatic analysis of lncRNA-mRNA interactions.
- Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis.
Main Results:
- Identified 4641 differentially expressed lncRNAs (3111 up-regulated, 1530 down-regulated) in MAP-infected cells.
- Predicted 86 lncRNAs targeting mRNAs, with 6 showing significant differential expression.
- Enriched pathways related to immune regulation, including NF-κB, NOD-like receptor, and Toll-like receptor signaling.
Conclusions:
- MAP infection significantly dysregulates lncRNA expression in bovine macrophages.
- The identified lncRNA-mRNA networks provide insights into immune responses to MAP.
- This study offers a foundation for understanding the molecular basis of paratuberculosis persistence.
