Plastid and cytoplasmic origins of ¹O₂-mediated transcriptomic responses

The reactive oxygen species singlet oxygen, ¹O₂, has an extremely short half-life, yet is intimately involved with stress signalling in the cell. We previously showed that the effects of ¹O₂ on the transcriptome are highly correlated with 80S ribosomal arrest due to oxidation of guanosine residues in mRNA. Here, we show that dysregulation of chlorophyll biosynthesis in the flu mutant or through feeding by δ-aminolevulinic acid can lead to accumulation of photoactive chlorophyll intermediates in the cytoplasm, which generates ¹O₂ upon exposure to light and causes the oxidation of RNA, eliciting ¹O₂-responsive genes. In contrast, transcriptomes derived from DCMU treatment, or the Ch1 mutant under moderate light conditions display commonalties with each other but do not induce ¹O₂ gene signatures. Comparing ¹O₂ related transcriptomes to an index transcriptome induced by cycloheximide inhibition enables distinction between ¹O₂ of cytosolic or of plastid origin. These comparisons provide biological insight to cases of mutants or environmental conditions that produce ¹O₂.