Janus electro-microenvironment membrane with surface-selective osteogenesis/gingival healing ability for guided bone...

Area of Science:

• Biomaterials Science
• Tissue Engineering
• Regenerative Medicine

Background:

• Guided bone regeneration (GBR) is crucial for treating alveolar bone defects.
• Severe defects exhibit slow healing and high rates of soft tissue dehiscence.
• Current GBR methods lack strategies for simultaneous, site-specific tissue healing.

Purpose of the Study:

• To develop a novel barrier membrane with a Janus electro-microenvironment (JEM) for side-selective bone and soft tissue regeneration.
• To investigate the underlying mechanisms of JEM-mediated tissue healing.
• To provide a new approach for managing complex craniofacial bone defects.

Main Methods:

• Fabrication of a polarized polyvinylidene fluoride ferroelectric membrane with distinct surface potentials (JEM- and JEM+).
• In vitro and in vivo assessment of osteogenic differentiation and bone formation on the JEM- side.
• Evaluation of soft tissue healing and gingival regeneration on the JEM+ side.
• Analysis of mitochondrial autophagy and oxidative phosphorylation pathways.

Main Results:

• The JEM membrane successfully promoted osteogenic differentiation and bone regeneration on the negatively polarized side (JEM-).
• The JEM- side enhanced bone formation via mitochondrial autophagy (LC3, Pink I, Parkin).
• The JEM+ side promoted gingival healing through oxidative phosphorylation (mitochondrial complexes I-V, ATP generation).

Conclusions:

• The JEM membrane offers a dual-function approach for guided bone regeneration and soft tissue healing.
• JEM- mediated bone regeneration through mitochondrial autophagy.
• JEM+ promoted soft tissue healing via enhanced mitochondrial oxidative phosphorylation.
• This JEM concept opens new possibilities for regulating regeneration at tissue interfaces.