Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

1.2K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
1.2K
Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

1.0K
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
1.0K
Mass Spectrometers01:16

Mass Spectrometers

5.9K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.9K
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

774
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
774
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

869
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
869
Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

677
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
677

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sustainable Imine Production Using Alkanes as Substrates via a Plasma-Microdroplet Approach.

Journal of the American Chemical Society·2025
Same author

Structural annotation of unknown molecules in a miniaturized mass spectrometer based on a transformer enabled fragment tree method.

Communications chemistry·2024
Same author

Diverse thermal desorption combined with self-aspirating corona discharge ionization for direct mass spectrometry analysis of complex samples.

Analytical methods : advancing methods and applications·2024
Same author

Hydrogen-Assisted Photoionization and Its Use in Promoting Mass Spectrometry Analysis of VOCs.

Analytical chemistry·2023
Same author

SR-Unet: A Super-Resolution Algorithm for Ion Trap Mass Spectrometers Based on the Deep Neural Network.

Analytical chemistry·2023
Same author

SWIFTSIN: A High-Resolution Ion Isolation Waveform for the Miniaturized Linear Ion Trap Mass Spectrometer by Coarse to Fine Excitation.

Analytical chemistry·2023

Related Experiment Video

Updated: Aug 20, 2025

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry
05:48

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry

Published on: September 5, 2014

9.7K

Micro-tapered aperture nebulization ionization for versatile mass spectrometry analysis.

Keguo Teng1, Jianbo Shi2, Yanping Zhu1

  • 1Division of Advanced Manufacturing, Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China. yu.quan@sz.tsinghua.edu.cn.

Analytical Methods : Advancing Methods and Applications
|November 24, 2022
PubMed
Summary

A novel micro-tapered aperture nebulization ionization (MANI) method offers voltage-free, gas-free analyte ionization for mass spectrometry. This simple, versatile MANI source enhances routine and diverse analytical applications.

More Related Videos

A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

11.3K
Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry
09:22

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Published on: September 3, 2010

14.3K

Related Experiment Videos

Last Updated: Aug 20, 2025

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry
05:48

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry

Published on: September 5, 2014

9.7K
A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

11.3K
Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry
09:22

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Published on: September 3, 2010

14.3K

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry Instrumentation

Background:

  • Advancements in ionization sources are crucial for expanding mass spectrometry applications.
  • Existing methods often require high voltage or additional gases, posing safety and complexity challenges.

Purpose of the Study:

  • To introduce and characterize a novel, high voltage-free ionization technique: micro-tapered aperture nebulization ionization (MANI).
  • To demonstrate the versatility and performance of the MANI source for various analytical tasks.

Main Methods:

  • Development of a compact MANI source by integrating a micro-tapered aperture atomizer into a 3D-printed chamber.
  • Characterization of the MANI source's ion yield and quantitative performance, particularly for aqueous solutions.

Main Results:

  • The MANI source successfully generates ions from liquid nebulization without external voltage or gas.
  • High ion yield and satisfactory quantitative performance were achieved, especially for aqueous samples.
  • Demonstrated broad applicability, including surface analysis and gaseous analyte detection via secondary spray ionization.

Conclusions:

  • MANI is a simple, safe, green, and versatile ionization technique.
  • The MANI source is a valuable tool for routine and diverse mass spectrometry analyses.
  • This method simplifies mass spectrometry by eliminating the need for high voltage and additional gases.