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Updated: Aug 20, 2025

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
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DNA methylation status of DNAJA4 is essential for human erythropoiesis

Hengchao Zhang1, Fumin Xue2, Huizhi Zhao1

  • 1School of Life Sciences, Zhengzhou University, Science Road 100, Zhengzhou, 450001, China.

Epigenomics
|November 24, 2022
PubMed

Aims: To investigate DNA methylation patterns in early and terminal stages of erythropoiesis, and to explore the function of differentially methylated genes in erythropoiesis and erythroid disorders. Materials & methods: Differential analysis of DNA methylation and gene expression during erythropoiesis, as well as weighted gene coexpression network analysis of acute myeloid leukemia was performed. Results: We identified four candidate genes that possessed differential methylation in the promoter regions. DNAJA4 affected proliferation, apoptosis and enucleation during terminal erythropoiesis and was associated with the prognosis of acute myeloid leukemia. DNAJA4 was specifically highly expressed in erythroleukemia and is associated with DNA methylation. Conclusion: DNAJA4 plays a crucial role for erythropoiesis and is regulated via DNA methylation. Dysregulation of DNAJA4 expression is associated with erythroid disorders.

Keywords:
DNA methylationDNAJA4acute myeloid leukemiagene expressionhuman erythropoiesisweighted coexpression network analysis

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