A Chemometric-Assisted Colorimetric-Based Inexpensive Paper Biosensor for Glucose Detection

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Materials Science

Background:

• Accurate glucose monitoring is crucial for managing diabetes and other metabolic disorders.
• Current methods can be complex, expensive, or require specialized equipment.
• Development of simple, low-cost diagnostic tools is needed for widespread accessibility.

Purpose of the Study:

• To develop a simple, inexpensive, and leak-proof paper pad for glucose detection.
• To establish a rapid colorimetric method for quantifying glucose concentrations.
• To optimize detection parameters for accuracy and reliability.

Main Methods:

• Selection of paper substrate based on surface morphology and fluid absorption.
• Application of a drying method for colorimetric glucose detection.
• Optimization of horseradish peroxidase (HRP) concentration and volume for spot detection.
• Chemometric analysis to investigate and optimize detection conditions.

Main Results:

• A leak-proof paper pad for glucose detection was successfully developed.
• A rapid colorimetric method achieved a limit of detection (LOD) of 2.92 mmol L^-1.
• The method demonstrated effective glucose detection in the range of 0.5-20 mM and 0.1-0.5 M.

Conclusions:

• The developed paper pad offers a simple and cost-effective approach for glucose detection.
• The method shows potential for initial screening of glucose levels in healthcare.
• The concept can be extended to create point-of-care testing devices for various diseases.