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Sample-to-Answer Immuno-Magnetic Assay Using Thermally Responsive Alkane Partitions.

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Rapid point-of-care diagnostics are crucial for pandemic response. Thermally responsive alkane partitions (TRAPs) enable sample-to-answer detection of SARS-CoV-2 antibodies in whole blood, aiding disease tracking.

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Area of Science:

  • Biotechnology
  • Immunodiagnostics
  • Pandemic Preparedness

Background:

  • Effective pandemic response requires rapid point-of-care diagnostics for patient identification and disease surveillance.
  • Current diagnostics need improvement for detecting biomarkers like antibodies in whole blood at the point of care.
  • Thermally Responsive Alkane Partitions (TRAPs) have been developed for automated immuno-magnetic assays.

Purpose of the Study:

  • To demonstrate the utility of TRAPs for a sample-to-answer immunoassay.
  • To detect antibodies against SARS-CoV-2 in whole blood samples using TRAP technology.
  • To establish the performance characteristics of this novel diagnostic approach.

Main Methods:

  • Development and application of TRAP technology for automated immuno-magnetic assays.
  • Utilizing TRAPs to enable direct detection of SARS-CoV-2 antibodies from whole blood.
  • Quantification of antibody levels and determination of the limit of detection.

Main Results:

  • Successful implementation of TRAP-enabled, sample-to-answer detection of SARS-CoV-2 antibodies.
  • Achieved a limit of detection of 84 pg/mL, which is clinically relevant.
  • Demonstrated the capability to analyze whole blood samples without prior processing.

Conclusions:

  • TRAP technology facilitates rapid, sensitive, point-of-care detection of viral antibodies.
  • This assay is suitable for tracking pandemic progression and informing clinical decisions.
  • TRAP-enabled immunoassays offer a promising tool for future pandemic preparedness and response.