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Mining Small Molecules from Teredinibacter turnerae Strains Isolated from Philippine Teredinidae
Jamaine B Villacorta1, Camille V Rodriguez1, Jacquelyn E Peran2
1Small Molecules Profiling Laboratory (SMPL), Institute of Chemistry, College of Science, University of the Philippines, Diliman, Quezon City 1101, Philippines.
Metabolites
|November 24, 2022
Summary
Shipworm endosymbionts produce diverse metabolites, including lipids and cyclic dipeptides, with potential antimicrobial activity. This study highlights the use of advanced metabolomics and bioinformatics for discovering novel marine natural products.
Area of Science:
- Marine Microbiology and Natural Products Chemistry
- Metabolomics and Bioinformatics
Background:
- Endosymbiotic relationships are crucial for marine species' evolution and metabolite synthesis.
- Shipworm endosymbionts are a source of novel biochemical compounds.
Purpose of the Study:
- To explore the chemical diversity of exogenous compounds from shipworm endosymbionts.
- To identify and characterize novel metabolites using advanced analytical techniques.
Main Methods:
- Liquid chromatography-mass spectrometry (LC-MS)-based metabolomics on cultured shipworm endosymbiont extracts.
- Ultrahigh-performance liquid chromatography with high-resolution mass spectrometry quadrupole time-of-flight mass analyzer (UHPLC-HRMS QTOF) for metabolite profiling.
- Global Natural Products Social (GNPS) molecular networking for dereplication and annotation of tandem MS data.
Main Results:
- Identified 93 clusters of metabolites, including lipids, lysophosphatidylethanolamines, cyclic dipeptides, and rhamnolipids.
- Putatively identified tartrolon D analogues, turnercyclamycins A and B, teredinibactin A, and oxylipins.
- Discovered additional clusters of lyso-ornithine lipids and polyethers, showcasing significant chemical diversity.
Conclusions:
- Shipworm endosymbionts harbor a diverse array of chemical compounds with potential pharmaceutical applications.
- Bioinformatics and molecular networking are effective tools for dereplicating high-throughput metabolomics data.
- Further research is needed to fully elucidate the structures of all identified marine natural products.
