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Rapid Bacterial Motility Monitoring Using Inexpensive 3D-Printed OpenFlexure Microscopy Allows Microfluidic

Tai The Diep1, Sarah Helen Needs1, Samuel Bizley1

  • 1Reading School of Pharmacy, University of Reading, Reading RG6 6AD, UK.

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Summary

A new 3D-printed microscope system uses microfluidic strips for rapid antibiotic susceptibility testing. This portable, inexpensive method detects bacterial motility changes to identify antimicrobial resistance, aiding global health efforts.

Keywords:
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Area of Science:

  • Microbiology
  • Biomedical Engineering
  • Medical Diagnostics

Background:

  • Antimicrobial resistance (AMR) is a growing global health threat requiring rapid diagnostic tools.
  • Traditional antibiotic susceptibility testing (AST) can be time-consuming and requires specialized equipment.
  • Portable, low-cost microscopy offers potential for decentralized diagnostic solutions.

Purpose of the Study:

  • To develop and evaluate a novel, inexpensive microdevice-miniature microscope system for multiplexed antibiotic susceptibility testing.
  • To assess the feasibility of using bacterial motility as a rapid indicator of antibiotic response.
  • To integrate a 3D-printed microscope with disposable microfluidic test strips for AST.

Main Methods:

  • Fabrication of a microdevice with parallel microcapillaries using melt-extruded plastic film.
  • Loading microcapillaries with Mueller-Hinton agar containing ceftazidime and gentamicin.
  • Utilizing a 3D-printed OpenFlexure digital microscope with a Raspberry Pi and CMOS camera for image acquisition.
  • Observing bacterial motility within antibiotic-loaded microcapillaries.

Main Results:

  • The system successfully detected bacterial motility inhibition in response to antibiotic exposure.
  • Sufficient image quality was achieved to differentiate effects of varying antibiotic concentrations.
  • The portable microscope platform demonstrated adequate magnification and field of view for motility assessment.

Conclusions:

  • A 3D-printed Raspberry Pi-based microscope combined with disposable microfluidic test strips enables rapid, user-friendly bacterial motility detection.
  • This system shows potential for aiding the detection of antibiotic resistance in resource-limited settings.
  • The developed platform offers a cost-effective alternative for point-of-care antibiotic susceptibility testing.