Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

186
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
186
Pulmonary Tuberculosis I01:29

Pulmonary Tuberculosis I

297
Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
297
Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

225
Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
225
Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

299
Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
299
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

412
Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
412

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Within-host pathogen population diversity predicts treatment response in Tuberculosis.

medRxiv : the preprint server for health sciences·2026
Same author

Convolutional neural networks quantify antibiotic resistance in Mycobacterium tuberculosis with diagnostic grade accuracy and predict treatment response.

Nature communications·2026
Same author

Tuberculosis Disease Severity Assessment Using Clinical Variables and Radiology Enabled by Artificial Intelligence.

The Journal of infectious diseases·2026
Same author

Gene conversion is a key driver of diversity hotspots in <i>M. tuberculosis</i> antigens and virulence-associated loci.

bioRxiv : the preprint server for biology·2026
Same author

Benchmarking within-sample minority variant detection with short-read sequencing in <i>M. tuberculosis</i>.

bioRxiv : the preprint server for biology·2026
Same author

Screening and targeted sequencing of stool for microbiologic confirmation and drug resistance determination in paucibacillary tuberculosis.

PLOS global public health·2025

Related Experiment Video

Updated: Aug 20, 2025

Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples
06:07

Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples

Published on: June 6, 2025

301

High Throughput Sequencing for Clinical Tuberculosis: An Overview.

Tara E Ness1,2, Andrew DiNardo2, Maha R Farhat3

  • 1Division of Pediatric Infectious Diseases, Baylor College of Medicine/Texas Children's Hospital, Houston, TX 77030, USA.

Pathogens (Basel, Switzerland)
|November 24, 2022
PubMed
Summary

High throughput sequencing (HTS) aids in diagnosing tuberculosis (TB) by detecting Mycobacterium tuberculosis DNA and predicting drug susceptibility. This review explores HTS applications, challenges, and innovations for TB diagnostics.

Keywords:
Mycobacterium tuberculosishigh throughput sequencingnext generation sequencingsequencingtuberculosis

More Related Videos

Author Spotlight: Optimizing CFU Determination for Efficient Assessment of TB Vaccine Efficacy and Antigen Presentation Analysis
06:26

Author Spotlight: Optimizing CFU Determination for Efficient Assessment of TB Vaccine Efficacy and Antigen Presentation Analysis

Published on: July 28, 2023

2.3K
Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
07:35

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

12.8K

Related Experiment Videos

Last Updated: Aug 20, 2025

Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples
06:07

Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples

Published on: June 6, 2025

301
Author Spotlight: Optimizing CFU Determination for Efficient Assessment of TB Vaccine Efficacy and Antigen Presentation Analysis
06:26

Author Spotlight: Optimizing CFU Determination for Efficient Assessment of TB Vaccine Efficacy and Antigen Presentation Analysis

Published on: July 28, 2023

2.3K
Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
07:35

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

12.8K

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • High throughput sequencing (HTS) offers a powerful tool for pathogen identification.
  • Tuberculosis (TB) diagnosis remains a critical global health challenge.
  • Current diagnostic methods for TB have limitations in speed and comprehensiveness.

Purpose of the Study:

  • To review the clinical applications of HTS for diagnosing Mycobacterium tuberculosis infections.
  • To analyze the strengths and limitations of various HTS platforms and analytical tools.
  • To provide a framework for future innovations in HTS-based TB diagnostics.

Main Methods:

  • Literature review of studies on HTS for TB diagnosis.
  • Analysis of workflow challenges, platform capabilities, and bioinformatics tools.
  • Synthesis of current evidence on HTS performance in clinical settings.

Main Results:

  • HTS can simultaneously detect Mycobacterium tuberculosis DNA and infer drug susceptibility.
  • Various HTS platforms exhibit different strengths and limitations regarding cost, speed, and sensitivity.
  • Bioinformatics tools are crucial for accurate data analysis and interpretation.

Conclusions:

  • HTS holds significant promise for revolutionizing TB diagnostics by enabling rapid, comprehensive analysis.
  • Addressing workflow challenges and optimizing analytical pipelines are key for widespread clinical adoption.
  • Further research and development are needed to fully realize the potential of HTS in TB management.