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PICLN modulates alternative splicing and light/temperature responses in plants
Julieta L Mateos1,2,3, Sabrina E Sanchez1, Martina Legris1,4
1Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires C1405BWE, Argentina.
Plant Physiology
|November 24, 2022
Summary
Plant adaptation to environmental changes involves pre-mRNA splicing regulation by the methylosome component Ion Chloride nucleotide-sensitive protein (PICLN). This splicing factor plays a crucial role in modulating plant responses to light and temperature fluctuations.
Area of Science:
- Plant molecular biology
- Gene expression regulation
- Environmental adaptation
Background:
- Plants adapt to environmental changes via transcriptome reprogramming.
- Splicing factors, particularly those in the methylosome complex, are increasingly recognized for their role in plant development and environmental responses.
- Ion Chloride nucleotide-sensitive protein (PICLN) is a conserved component of the methylosome complex in both humans and Arabidopsis.
Purpose of the Study:
- To investigate the role of Ion Chloride nucleotide-sensitive protein (PICLN) in plant adaptation to environmental conditions.
- To determine if human PICLN can rescue Arabidopsis picln mutants.
- To elucidate the molecular mechanisms by which PICLN influences pre-mRNA splicing and plant responses.
Main Methods:
- Phenotypic analysis of Arabidopsis picln mutants.
- Complementation of Arabidopsis picln mutants with human PICLN ortholog.
- Analysis of pre-mRNA splicing patterns in wild-type and mutant plants.
- Investigating the interaction of PICLN with other splicing factors like GEMIN2.
- Assessing the impact of low temperature on picln mutant phenotypes and gene expression.
Main Results:
- Human PICLN ortholog rescues phenotypes of Arabidopsis picln mutants.
- Arabidopsis picln mutants exhibit altered photomorphogenic and photoperiodic responses due to changes in pre-mRNA splicing.
- Splicing changes in picln mutants partially overlap with those in PROTEIN ARGININE METHYL TRANSFERASE5 (PRMT5) mutants.
- PICLN-regulated splicing events, distinct from PRMT5, are controlled by GEMIN2 in Arabidopsis.
- Low temperature exacerbates morphological and molecular defects in picln mutants, correlating with increased PICLN expression.
Conclusions:
- PICLN is essential for regulating pre-mRNA splicing in plants.
- PICLN plays a key role in mediating plant adaptation to daily and seasonal environmental fluctuations.
- PICLN functions in conjunction with GEMIN2 to control specific alternative splicing events.
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