The noncoding RNA HIDDEN TREASURE 1 promotes phytochrome B-dependent seed germination by repressing abscisic acid
Yuqiu Wang1, Yangyang Fan1, De Fan1,2
1State Key Laboratory of Protein and Plant Gene Research, School of Advanced Agricultural Sciences and School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.
The Plant Cell
|November 24, 2022
Summary
Red light signaling via phytochrome B (phyB) promotes seed germination by regulating phytohormones. This study identifies HIDDEN TREASURE 1 (HID1) as a key noncoding RNA repressor of abscisic acid (ABA) biosynthesis, crucial for this process.
Area of Science:
- Plant Biology
- Molecular Biology
- Genetics
Background:
- Seed germination is critically regulated by environmental factors like light.
- Phytochrome B (phyB) mediates red light responses, influencing seed germination by balancing gibberellic acid (GA) and abscisic acid (ABA).
- The precise mechanism by which phyB modulates ABA biosynthesis post-light perception remains unclear.
Purpose of the Study:
- To identify novel factors downstream of phyB involved in regulating ABA biosynthesis during seed germination.
- To elucidate the role of the noncoding RNA HIDDEN TREASURE 1 (HID1) in phyB-mediated light signaling.
- To understand how HID1 interacts with ABA biosynthesis pathways and epigenetic modifiers.
Main Methods:
- Forward genetic screening in Arabidopsis thaliana.
- Transcriptomics analysis to identify co-regulated genes.
- RNA immunoprecipitation (RIP) and chromatin immunoprecipitation (ChIP) assays.
- Analysis of gene expression and protein-protein interactions.
Main Results:
- HIDDEN TREASURE 1 (HID1), a noncoding RNA, was identified as a repressor of ABA biosynthesis acting downstream of phyB.
- Loss of HID1 function resulted in delayed phyB-dependent seed germination.
- Photoactivated phyB increased HID1 accumulation, which co-regulated ABA and GA metabolism genes with phyB.
- HID1 directly inhibits the transcription of 9-CIS-EPOXYCAROTENOID DIOXYGENASE (NCED9), a key ABA biosynthesis enzyme.
- HID1 interacts with ARABIDOPSIS TRITHORAX-RELATED7 (ATXR7) to suppress H3K4me3 modification at the NCED9 locus.
Conclusions:
- HID1 acts as a nuclear mediator of phyB signaling, repressing ABA biosynthesis.
- This mechanism involving HID1 and ATXR7 fine-tunes ABA homeostasis, essential for optimizing the transcriptional network during seed germination.
- The study reveals a novel nuclear pathway linking light perception to hormonal balance for germination control.
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