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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Updated: Aug 20, 2025

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
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CRISPR/Cas technology: Opportunities for phytopathogenic viruses detection.

Dinesh Prasad1, Naresh Kumar Mani2, Dev Mani Pandey1

  • 1Department of Bioengineering and Biotechnology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand 835215, India.

Journal of Biotechnology
|November 24, 2022
PubMed
Summary

CRISPR/Cas technology offers promising new methods for detecting plant viruses, crucial for agricultural health. These advanced diagnostic tools, though nascent, hold potential for cost-effective, broad-spectrum virus detection in agriculture.

Keywords:
CRISPR/Cas systemDETECTRDiagnosticsSATORISHERLOCKViral pathogen

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Area of Science:

  • Agricultural Science
  • Molecular Biology
  • Biotechnology

Background:

  • Virus detection is critical for plant health and agricultural productivity.
  • The diversity of plant viruses poses challenges for developing universal detection methods.
  • CRISPR/Cas systems are emerging as powerful tools for pathogen diagnostics.

Purpose of the Study:

  • To review the application of CRISPR/Cas technology for diagnosing plant viruses.
  • To highlight specific CRISPR-based diagnostic methods like SHERLOCK, DETECTR, and SATORI.
  • To discuss the potential of these technologies for commercial development in agriculture.

Main Methods:

  • Review of literature on CRISPR/Cas-based diagnostic systems for plant pathogens.
  • Analysis of different CRISPR-associated proteins and their diagnostic applications.
  • Examination of nucleic acid detection and signal processing in SHERLOCK, DETECTR, and SATORI.

Main Results:

  • CRISPR/Cas technology provides diverse strategies for specific nucleic acid identification.
  • Methods like SHERLOCK, DETECTR, and SATORI demonstrate potential for sensitive virus detection.
  • These technologies are currently in early stages of development with room for commercialization.

Conclusions:

  • CRISPR/Cas-based diagnostics offer a promising avenue for efficient and potentially cost-effective plant virus detection.
  • Further development is needed to create commercial kits applicable to the diverse range of phytopathogenic viruses.
  • Successful implementation could significantly benefit agro-based industries by mitigating virus contamination challenges.