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Related Experiment Video

Updated: Aug 20, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Multimodal single-molecule microscopy with continuously controlled spectral resolution.

Jonathan Jeffet^1,2,3, Ariel Ionescu^4,5, Yael Michaeli^1,2

¹Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, Israel.

Biophysical Reports

|November 25, 2022

Summary

This summary is machine-generated.

Continuously controlled spectral-resolution (CoCoS) microscopy enables real-time adjustment of spectral resolution. This advance enhances sensitivity and throughput for multicolor fluorescence imaging and spectroscopy.

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Last Updated: Aug 20, 2025

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Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

Area of Science:

Optics and Photonics
Biophysics
Microscopy

Background:

Color is crucial for contrast in fluorescence microscopy, underpinning various imaging and spectroscopy methods.
Existing spectral imaging techniques encode color into fixed spatial distributions, limiting flexibility.
Super-resolution spectral imaging has advanced but faces limitations in field of view and throughput.

Purpose of the Study:

To introduce continuously controlled spectral-resolution (CoCoS) microscopy, a novel technique for real-time spectral resolution adjustment.
To demonstrate CoCoS microscopy's capability for enhanced sensitivity and throughput in fluorescence imaging.
To make advanced spectral imaging more accessible to researchers.

Main Methods:

Development of CoCoS microscopy with adjustable spectral resolution.
Integration of CoCoS as a simple add-on module for existing fluorescence microscopes.
Application of CoCoS in single-molecule spectroscopy, Förster resonance energy transfer, and multicolor single-particle tracking.

Main Results:

CoCoS microscopy allows real-time optimization of spectral resolution for specific experiments.
Achieved single-frame acquisition of multiple color channels with single-molecule sensitivity.
Demonstrated a 140-fold increase in field of view compared to previous super-resolution spectral imaging techniques.
Successfully applied CoCoS to live neurons and diverse fluorescent markers.

Conclusions:

CoCoS microscopy offers significant improvements in sensitivity, throughput, and field of view for spectral imaging.
The technique is versatile, applicable to various fluorescence-based analyses including live-cell imaging.
CoCoS microscopy provides an accessible upgrade for existing fluorescence microscopes, broadening the reach of spectral imaging.