Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Lineage Commitment

Lineage Commitment

Commitment is the process whereby stem cells:

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

Differentiation of Common Myeloid Progenitor Cells

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...

T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

Cells of the Adaptive Immune Response

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Altered Thymopoiesis in Thymoma Is Associated with Defects in Negative Selection Machinery and Decreased Treg Abundance.

Cancer immunology research·2025

Same author

Origin and evolutionary trajectories of brown algal sex chromosomes.

Nature ecology & evolution·2025

Same author

Ferritin heavy chain supports stability and function of the regulatory T cell lineage.

The EMBO journal·2024

Same author

A conserved transcriptional program for MAIT cells across mammalian evolution.

The Journal of experimental medicine·2023

Same author

Peptidylprolyl isomerase C (Ppic) regulates invariant Natural Killer T cell (iNKT) differentiation in mice.

European journal of immunology·2021

Same author

Self-renewal of double-negative 3 early thymocytes enables thymus autonomy but compromises the β-selection checkpoint.

Cell reports·2021

Search research articles

Related Experiment Video

Updated: Aug 20, 2025

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

IL-7 Receptor Drives Early T Lineage Progenitor Expansion.

Rafael A Paiva¹, Camila V Ramos¹, Gonçalo Leiria²

¹Lymphocyte Development and Leukemogenesis Laboratory, Instituto Gulbenkian de Ciência, Calouste Gulbenkian Foundation, Oeiras, Portugal; and.

Journal of Immunology (Baltimore, Md. : 1950)

|November 25, 2022

Summary

Interleukin-7 (IL-7) and its receptor (IL-7R) are crucial for the survival and proliferation of early T lineage progenitors (ETPs). This study demonstrates IL-7/IL-7R signaling is essential for the earliest T-cell development stages in the thymus.

More Related Videos

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Published on: June 9, 2015

Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia

Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia

Published on: June 9, 2023

Related Experiment Videos

Last Updated: Aug 20, 2025

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Published on: June 9, 2015

Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia

Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia

Published on: June 9, 2023

Area of Science:

Immunology
Developmental Biology
Cell Biology

Background:

Interleukin-7 (IL-7) and its receptor (IL-7R) are known regulators of T lymphocyte differentiation.
Early T lineage progenitors (ETPs) express IL-7R, but its necessity at this immature stage remains unclear.

Purpose of the Study:

To investigate the role of IL-7 and IL-7R in the proliferation and survival of ETPs.
To determine if IL-7R signaling is required for the earliest stages of T-cell development.

Main Methods:

Analysis of mice deficient in IL-7 or IL-7R.
Ex vivo stimulation of ETPs with IL-7 and assessment of Stat5 phosphorylation.
In vitro coculture systems (OP9-DLL4) to study ETP expansion and differentiation.

Main Results:

Mice lacking IL-7 or IL-7R exhibited a significant reduction in ETPs due to impaired proliferation and survival.
ETPs responded to IL-7 by phosphorylating Stat5, with IL-7R enriched in the most immature Flt3+Ccr9+ ETPs.
IL-7 promoted the expansion of Flt3+ ETPs in vitro without affecting differentiation.

Conclusions:

IL-7/IL-7R signaling is essential for the proliferation and survival of the most immature thymocytes after thymus seeding.
This pathway is critical for maintaining the ETP pool, thereby supporting T-cell development.