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Fabricated polyhydroxyalkanoates blend scaffolds enhance cell viability and cell proliferation
Sunena Dhania1, Ruma Rani2, Rajender Kumar2
1Department of Bio & Nanotechnology, Guru Jambheshwar University of Science and Technology, Hisar 125001, Haryana, India.
Journal of Biotechnology
|November 25, 2022
Summary
Blended poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) scaffolds enhance cell adhesion and proliferation for tissue engineering. These biodegradable materials offer improved porosity, surface area, and hydrophilicity, promoting better cell viability.
Area of Science:
- Biomaterials Science
- Polymer Science
- Tissue Engineering
Background:
- Cell adhesion and proliferation are critical for successful tissue engineering scaffolds.
- Polymer blending offers a versatile strategy to tailor scaffold properties for biocompatibility and functionality.
Purpose of the Study:
- To develop and characterize novel porous, biodegradable scaffolds by blending PHB and PHBV.
- To evaluate the physical, chemical, and biological properties of these blended scaffolds for tissue engineering applications.
Main Methods:
- Polymer blending of PHB and PHBV in various ratios using the salt leaching technique.
- Scaffold characterization via SEM, BET, FTIR, and water contact angle measurements.
- Hemocompatibility assessment using a hemolysis assay and cell viability/attachment studies with Vero, Hela, and MDBK cell lines.
Main Results:
- Blended scaffolds exhibited reduced crystallinity, varied porosity, increased surface area, and enhanced hydrophilicity.
- Hemolysis assays confirmed hemocompatibility, with no adverse effects on red blood cells.
- Significant improvements in cell viability, proliferation, and attachment were observed for multiple cell lines cultured on the blended scaffolds.
Conclusions:
- PHB/PHBV blended scaffolds demonstrate promising characteristics for tissue engineering applications.
- The enhanced physical and biological properties of these scaffolds support their potential use in regenerative medicine.
