Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Signaling01:30

Bacterial Signaling

33.5K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
33.5K
Coordination of Gene Expression Processes in Bacteria01:29

Coordination of Gene Expression Processes in Bacteria

124
The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
124
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

69
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
69
Global Regulatory Systems01:28

Global Regulatory Systems

60
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
60
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

62
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
62
Bacterial Protein Maturation01:26

Bacterial Protein Maturation

63
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
63

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discovery of a mitochondria-targeted propranolol conjugate PL37 that induces mitophagy for potent anti-hemangioma activity.

Bioorganic & medicinal chemistry·2026
Same author

Design, synthesis, and biological evaluation of a multi-layer-linked idebenone derivative targeting mitochondrial dysfunction and neuroinflammation for ischemic stroke.

Bioorganic chemistry·2026
Same author

Localizable Fluorescent Metal Ion Indicators With Tunable Colors.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

A high-affinity split-HaloTag for live-cell protein labeling.

Nature communications·2026
Same author

Biocompatible ligand balancing in transition metal coordination enables benign in-cell protein arylation.

Nature chemistry·2026
Same author

A mitochondria-targeted idebenone derivative W1a mitigates cerebral ischemia-reperfusion injury by preserving mitochondrial integrity and suppressing neuroinflammation.

Bioorganic chemistry·2025

Related Experiment Video

Updated: Aug 20, 2025

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
11:33

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking

Published on: December 17, 2013

6.3K

Interbacterial Chemical Communication-Triggered Nascent Proteomics.

Weibing Liu1, Qi Tang1, Liying Meng1,2

  • 1College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, and Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871, China.

Angewandte Chemie (International Ed. in English)
|November 25, 2022
PubMed
Summary

A new method, CAPPEX, tracks protein synthesis during bacterial communication. It revealed how indole, produced via tryptophan competition between E. coli and Salmonella, reduces Salmonella virulence.

Keywords:
Colonization ResistanceInterbacterial CommunicationMetabolic Protein LabelingNascent Proteomics

More Related Videos

Using Coculture to Detect Chemically Mediated Interspecies Interactions
08:29

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Published on: October 31, 2013

13.7K
Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response
06:26

Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response

Published on: May 23, 2020

8.3K

Related Experiment Videos

Last Updated: Aug 20, 2025

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
11:33

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking

Published on: December 17, 2013

6.3K
Using Coculture to Detect Chemically Mediated Interspecies Interactions
08:29

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Published on: October 31, 2013

13.7K
Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response
06:26

Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response

Published on: May 23, 2020

8.3K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Systems Biology

Background:

  • Nascent proteome profiling using metabolic labeling with clickable noncanonical amino acids allows cell-type-specific analysis.
  • Investigating intercellular communication-dependent nascent proteomics remains a significant challenge.

Purpose of the Study:

  • To develop a novel method, Communication-Activated Profiling of Protein Expression (CAPPEX), for studying nascent protein synthesis in response to intercellular signals.
  • To investigate the role of interbacterial communication in regulating bacterial virulence and host-pathogen interactions.

Main Methods:

  • CAPPEX integrates the LuxI/LuxR quorum sensing circuit with cell-type-specific nascent proteomics.
  • This enables selective click-labeling of newly synthesized proteins in response to specific chemical signals from reporter bacteria.

Main Results:

  • CAPPEX identified competition between E. coli and Salmonella for tryptophan, a precursor for indole production.
  • The study found that indole suppresses the expression of virulence factors in Salmonella.
  • This tryptophan-indole axis was shown to attenuate Salmonella invasion in host cells and living mice.

Conclusions:

  • CAPPEX provides a powerful strategy for dissecting interbacterial communication networks and their impact on bacterial behavior.
  • The findings highlight a novel mechanism of virulence regulation in Salmonella mediated by interbacterial signaling.