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Enzyme-Linked Immunosorbent Assay

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Related Experiment Video

Updated: Aug 20, 2025

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

Lateral Flow Immunoassays for SARS-CoV-2.

Geik Yong Ang¹, Kok Gan Chan^2,3, Chan Yean Yean⁴

¹Faculty of Sports Science and Recreation, Universiti Teknologi MARA, Shah Alam 40450, Malaysia.

Diagnostics (Basel, Switzerland)

|November 26, 2022

Summary

Lateral flow immunoassays (LFAs) offer a rapid, low-cost, and equipment-free diagnostic solution for SARS-CoV-2, crucial for low-resource settings. These tests detect viral antigens or host immune responses, aiding in managing the transition from pandemic to endemic COVID-19.

Keywords:

antibody antigen diagnostic dipstick immunochromatography immunosensor point-of-care serology

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Last Updated: Aug 20, 2025

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

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Published on: April 6, 2021

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Published on: February 16, 2022

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

Area of Science:

Infectious Diseases
Biotechnology
Medical Diagnostics

Background:

SARS-CoV-2 continues to circulate globally, posing risks of more virulent variants and potential epidemic waves.
Nucleic acid-based diagnostic tests for COVID-19 face limitations in accessibility and affordability in low-resource settings due to equipment requirements.

Purpose of the Study:

To review the principles, formats, and performance of lateral flow immunoassays (LFIs) for SARS-CoV-2 detection.
To highlight LFIs as a viable alternative to nucleic acid tests in resource-limited environments.
To discuss specimen types, marker selection, and challenges in LFI applications for COVID-19.

Main Methods:

Review of emergency use authorized LFIs by the US Food and Drug Administration.
Analysis of LFI principles, assay formats, specimen types, and marker selection.
Evaluation of LFI performance characteristics.

Main Results:

LFIs are appealing due to their rapid, low-cost, equipment-free, and scalable nature, suitable for point-of-care settings.
LFIs can detect SARS-CoV-2 antigens or measure host immune responses.
The review emphasizes LFIs granted emergency use authorization, detailing their performance.

Conclusions:

LFIs present a highly accessible and practical diagnostic tool for SARS-CoV-2, particularly in resource-limited settings.
Addressing challenges and exploring future perspectives is crucial for optimizing LFI applications in infectious disease management.
LFIs are vital for managing the ongoing threat of SARS-CoV-2 variants and the transition to endemicity.