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Updated: Aug 20, 2025

Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Coordination Chemistry of Phosphate Groups in Systems Including Copper(II) Ions, Phosphoethanolamine and Pyrimidine
Malwina Gabryel-Skrodzka1, Martyna Nowak1, Anna Teubert2
1Faculty of Chemistry, Adam Mickiwicz University in Poznan, Uniwersytetu Poznanskiego 8, 61-614 Poznań, Poland.
Abstract:
The activity of phosphate groups of phosphoethanolamine and pyrimidine nucleotides (thymidine 5-monophosphate, cytidine 5-monophosphate and uridine 5'monophosphate) in the process of complexation metal ions in aqueous solution was studied. Using the potentiometric method with computer calculation of the data and spectroscopic methods such as UV-Vis, EPR, 13C and 31P NMR as well as FT-IR, the overall stability constants of the complexes as well as coordination modes were obtained. At lower pH, copper(II) ions are complexed only by phosphate groups, whereas the endocyclic nitrogen atom of nucleotides has been identified as a negative center interacting with the -NH3+ groups of phosphoethanolamine.
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