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Updated: Aug 19, 2025
12:04
Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
Published on: June 24, 2019
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Fission Yeast Rho1p-GEFs: From Polarity and Cell Wall Synthesis to Genome Stability
Patricia García1, Rubén Celador1, Jorge Pérez-Parrilla1
1Instituto de Biología Funcional y Genómica, CSIC/Universidad de Salamanca and Departamento de Microbiología y Genética, Universidad de Salamanca, C/Zacarías González, s/n, 37007 Salamanca, Spain.
International Journal of Molecular Sciences
|November 26, 2022
Summary
Fission yeast Rho1p guanine nucleotide exchange factors (GEFs) Rgf1p, Rgf2p, and Rgf3p regulate distinct cellular processes. These GEFs also play noncanonical roles in maintaining genomic stability.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- Rho1p, a small GTPase, is crucial for yeast cell shape and homeostasis.
- It cycles between active (GTP-bound) and inactive (GDP-bound) states, regulated by GEFs and GAPs.
- Fission yeast Rho1p GEFs, Rgf1p, Rgf2p, and Rgf3p, are Dbl-related proteins with DH-PH domains.
Purpose of the Study:
- To review the functions of fission yeast Rho1p GEFs: Rgf1p, Rgf2p, and Rgf3p.
- To highlight their roles in specific cellular processes and their noncanonical functions.
- To explore their involvement in genomic instability.
Main Methods:
- Literature review of Rho1p and its GEFs in fission yeast.
- Analysis of studies detailing the functions of Rgf1p, Rgf2p, and Rgf3p.
- Examination of research on Rho1p-GEF roles in cellular processes and genomic stability.
Main Results:
- Rgf1p is primarily involved in polarized growth.
- Rgf2p is essential for sporulation.
- Rgf3p plays a critical role in septum synthesis.
- Rho1p-GEFs exhibit noncanonical roles in genomic instability.
Conclusions:
- Rgf1p, Rgf2p, and Rgf3p differentially regulate Rho1p activity in specific cellular contexts.
- These GEFs are vital for distinct aspects of yeast cell biology, including growth, reproduction, and division.
- Emerging evidence suggests Rho1p-GEFs have significant roles in maintaining genomic integrity.
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